Molecular Identification Of Ukg Isolate And Characterization Of Its Inulin-Degrading Enzyme

Inulin-degrading bacteria from the dahlia tuber rhizosphere are potential sources of biocatalyst that hydrolyzed
inulin to produce fructose and fructooligosaccharide (FOS). The research was done to identify the UKG isolate
that screened from the dahlia tuber rhizosphere and determine the characterization of its extracellular inulin
degrading enzyme. The enzyme was purified using ammonium sulfate. Enzyme activity was determined using
DNS reagent. Inulin degrading enzyme from Klebsiella variicola UKG isolate worked optimally at pH 5 and
40ºC. The enzyme showed endo-type action. The estimated molecular mass of the inulin degrading enzyme was
43 kDa. The enzyme had KM 63,317 mg/mL, and Vmax 0,274 µmol/mL.min. The enzyme is suitable for the
production of FOS prebiotic from inulin.