Fractionation and Characterization of Proteins in Lumbricus rubellus Powders

Syamsurizal, [et.al] (2015) Fractionation and Characterization of Proteins in Lumbricus rubellus Powders. International Journal of Pharmaceutical and Clinical Research, 8 (1). pp. 15-21. ISSN 0975-1556

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Abstract

In order to find drugs from natural materials, earthworm powders Lumbricus rubellus can be used as a source of protein which can be developed into various types of drugs. The purpose of this study do fractionation and characterization of protein in L rubellus powders based on molecular weight. Protein in L rubellus powders extracted gradually to obtain a crude extract, precipitate and dialysate as final extract. Measurement of proteins levels in the crude extract, precipitate and dialysate is done by spectrophotometer, while electrophoresis was done to proteins characterization base on molecular weight. We conclude that 5 grams powder L rubellus in the dialysate fractions containing 2487 ug/mL proteins that show four dominant types of proteins with a molecular weight of 12.2, 13.3, 14.6 and 29.2 kiloDalton (kDa)

Item Type: Article
Subjects: R Medicine and Food > Sport General
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Pendidikan Biologi - S1
Depositing User: Mrs. Wiwi Sartika
Date Deposited: 25 Jan 2017 02:31
Last Modified: 20 Mar 2017 23:56
URI: http://repository.unp.ac.id/id/eprint/642

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