Genotyping SNP rs290487 TCF7L2 Gene Using T-Arms-PCR for Detection T2DM in Minangkabau Ethnic

Syamsurizal, Syamsurizal and Sardi, Arif and Kadri, Husni (2015) Genotyping SNP rs290487 TCF7L2 Gene Using T-Arms-PCR for Detection T2DM in Minangkabau Ethnic. In: International Conference on Bosciences Appreciating Unity in Diversity, 5-7 Agustus 2015, Bogor.

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Abstract

very important for the treatment efficiency and prevention of the disease. Previous studies showed that polymorphisms in TCF7L2 gene (SNP rs290487) associated with the possibility of T2DM in various ethnic groups in the world. The presence of the T allele in rs290487 can be used as a genetic marker of increased susceptibility to T2DM. The aim of this research was to find an association between SNP rs290487 with T2DM in ethnic Minangkabau. Furthermore, it is also useful for development of an T2DM detection method based on molecular technique are rapid, inexpensive and accurate. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using T-ARMS-PCR. The association between rs290487 with the possibility of T2DM known by chi-square test. T-ARMSPCR analysis show that the frequency of SNP rs290487 in 66 subject with T2DM was 42,4% (7,6% homozygote TT and 34,8% heterozygote TC) and for non-T2DM subject was 37,9% (7,6% homozygote TT and 30,3% heterozygote TC). There was no significant association between rs290487 SNP with the probability of T2DM in ethnic Minangkabau (P value =0.96).

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