Molecular Identification of UKG Isolate and Characterization of ITS Inulin-Degrading Enzyme

Azhar, Minda and Khairani, Syamsi and Putra, Ahadul and Daniel, Ike Ramadhanty and Ahda, Yuni and Ihsanawati, Ihsanawati and Puspitasari, Fernita and Natalia, Dessy (2021) Molecular Identification of UKG Isolate and Characterization of ITS Inulin-Degrading Enzyme. Rasayan J. Chem, 14 (1). pp. 171-178. ISSN ISSN: 0974-1496 | e-ISSN: 0976-0083

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Abstract

Inulin-degrading bacteria from the dahlia tuber rhizosphere are potential sources of biocatalyst that hydrolyzed inulin to produce fructose and fructooligosaccharide (FOS). The research was done to identify the UKG isolate that screened from the dahlia tuber rhizosphere and determine the characterization of its extracellular inulindegrading enzyme. The enzyme was purified using ammonium sulfate. Enzyme activity was determined using DNS reagent. Inulin degrading enzyme from Klebsiella variicola UKG isolate worked optimally at pH 5 and 40ºC. The enzyme showed endo-type action. The estimated molecular mass of the inulin degrading enzyme was 43 kDa. The enzyme had K M 63,317 mg/mL, and V max 0,274 µmol/mL.min. The enzyme is suitable for the production of FOS prebiotic from inulin

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