Characterization of extracellular enzyme and identification of Inulin degrading bacteria from hot springs in West Sumatra, Indonesia

Azhar, Minda and Syukur, Sumaryati and Natalia, Dessy and Vovien, Vovien and Jamsari, Jamsari and Minaf, Edison (2013) Characterization of extracellular enzyme and identification of Inulin degrading bacteria from hot springs in West Sumatra, Indonesia. International Journal of Chemistry, 2 (1). pp. 33-41. ISSN 2249-2119

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Abstract

Thermophilic bacteria are potential sources of inulin degrading thermostable enzyme, an enzyme which converts inulin into fructose and FOS prebiotics. Isolation and identification of inulin degrading bacteria from Bukik Kili hot springs in Solok, West Sumatra have been undertaken. Screening of inulin degrading bacteria was done using direct and indirect methods on medium with inulin or inulin-RBB as a sole carbon source. One inulin degrading bacterial isolate has been obtained from 17 isolates. The isolate was designated as UBCT-007. The isolate was able to grow at temperatures of 23°C to 60°C on medium with inulin as a sole carbon source. Optimal temperature and pH of extracellular enzyme on inulin substrate were 60ºC and 4.5 respectively. Thin layer chromatography (TLC) analysis of the end degradation product of inulin by extracellular enzyme from UBCT-007 bacterial isolate indicated exo-type action mode. The UBCT007 isolate was identified as Bacillus genus and the group of species was Bacillus licheniformis based on 16S rRNA gene, colony morphology, microscopic cell and biochemical test.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia - S1
Depositing User: Mrs. Wiwi Sartika
Date Deposited: 16 Sep 2016 03:27
Last Modified: 16 Sep 2016 03:27
URI: http://repository.unp.ac.id/id/eprint/459

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