Tetra Primer-Arms-Pcr Construction to Detect Snp Rs290487 Tcf7l2

Syamsurizal, Syamsurizal (2015) Tetra Primer-Arms-Pcr Construction to Detect Snp Rs290487 Tcf7l2. In: Education, mathematics, Science and Technology for Human and natural Resources, 22 Oktober 2015, Padang.

3. Proceding International ICOMSET.pdf

Download (6MB) | Preview


A Gene which reported to be associated with the expression of diabetes mellitus type-2 is Transcription Factor 7 Like 2 (TCF7L2). TCF7L2 gene have many polymorphisms (SNP). One of TCF7L2 gene polymorphisms that mostly study to detect diabetes mellitus is SNP rs290487. The objective of the research is: primer construction to detect SNP rs290487 TCF7L2. The main steps of the research are: DNA isolation, design tetra primers for SNP rs290487 TCF7L2 with primer designer software, SNP rs290487 TCF7L2 gene amplification with Tetra Primer-ARMS-PCR method, direct DNA sequencing method and bioinformatics analysis.Based on the data analysis, can be concluded that four primer has successfully constructed, and they are FOPRS29, ROPRS29, FIPRS29 and RIPRS29. FOPRS29 and ROPRS29 are external primer which amplify the fragments of the gene TCF7L2 contain SNP rs290487 (the size band that formed is 443 bp), then FIPRS29 used to amplify allele C (PCR product size is 208 bp) and RIPRS29 primer used to amplify the T allele with the size of the product is 293 bp. That constructed primer can recognize SNP rs290487 TCF7L2 when using Tetra Primer-ARMS-PCR Method

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Pendidikan Biologi - S1
Depositing User: Mrs. Wiwi Sartika
Date Deposited: 25 Jan 2017 00:38
Last Modified: 25 Jan 2017 00:38
URI: http://repository.unp.ac.id/id/eprint/635

Actions (login required)

View Item View Item