Yanti, Iren Gusni and Azhar, Minda and Faridah, Anni and Oktavia, Budhi (2019) Simple Determination of Inulin Polymerization Degree Average from Dahlia Tuber Using Spectrophotometer. International Journal of Research and Review (IJRR), 6 (8). pp. 399-404. ISSN Print: 2349-9788 dan Online: 2454-2237
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Abstract
Degree of polymerization (DP) of inulin is the number of monomer units in inulin polymer strand. DP of inulin can be determined simply using spectrophotometer. This study aims to determine DP of inulin from fresh dahlia tubers and dahlia tubers that stored for fifteen days. Inulin extraction from dahlia tuber was based on the solubility of inulin in hot water and precipitation in ethanol by adding ethanol to final concentration of 80% solution. Reducing sugars of inulin was determined using the DNS method. The absorbances were measured using spectrophotometer at maximum wavelength of 490 nm. Total sugar of inulin was determined using Phenol-sulphate method, the absorbance was measured at λ max 350 nm. Inulin from fresh dahlia tubers had higher DP than inulin from dahlia tuber that stored for fifteen days. Inulin that found in this research belongs to the fructooligosaccharide (FOS) group.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia - S1 |
| Depositing User: | Mrs. Wiwi Sartika |
| Date Deposited: | 01 Mar 2021 07:15 |
| Last Modified: | 01 Mar 2021 07:15 |
| URI: | http://repository.unp.ac.id/id/eprint/30231 |
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