The Addition of Various Carbon Sources on Growing Media to Increase the Siderophore Level of Fluorescent Pseudomonad Bacteria

Advinda, Linda and Pratama, Ilham and Fifendy, Mades and Anhar, Azwir and Armaleni, Armaleni (2018) The Addition of Various Carbon Sources on Growing Media to Increase the Siderophore Level of Fluorescent Pseudomonad Bacteria. In: ICOMSET2018: IOP Conf. Series: Journal of Physics: Conf. Series 1317 (2019) 012078, 2018.

Preview

Text addition of resources carbon.pdf Download (505kB) | Preview

Abstract

Fluorescent pseudomonad is a member of the rhizobacterial groups which has the potential to be developed as an agent for inducing plant resistance. Several species of fluorescent pseudomonad are capable of producing siderophore. Siderophore is an organic antimicrobial compound that plays a role in biological control of plant diseases. The aim of the study was to determine the best carbon source added to the growing media to increase the levels of siderophore from the fluorescent pseudomonad PfCas3 and PfLAHp2 isolates. This research is an experimental study that use a completely randomized design with 4 treatments, which is the addition of carbon sources of glucose, fructose, glycerol on growing media, and King's B medium (control), and 3 replications. Detection of siderophore was measured with a spectrophotometer at a 410 nm wavelength (λ). The data obtained were analyzed using ANOVA and DNMRT continued testing at a significant level of 5%. The results showed that the best carbon source added to the fluorescent pseudomonad PfCas3 isolate growing medium was glucose, with the siderophore level produced 1,574 at λ = 410 nm. Glucose added to the growing medium can also increase the levels of siderophore from the fluorescent pseudomonad PfLAHp2 isolate, which is 1,464 at λ = 410 nm

Actions (login required)

View Item